2GGI

The mutant E149C-A182C of Deinococcus Radiodurans N-acylamino acid racemase

Replaces:  2FKSReplaces:  2BA7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	2004-12-19
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 4
Unit cell lengths	116.504, 116.504, 120.685
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.200
R-factor	0.16829
Rwork	0.166
R-free	0.20251
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1r0m
RMSD bond length	0.011
RMSD bond angle	1.281
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	81711
Completeness [%]	99.1	98.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	lithium sulfate, Tris-HCl, PEG4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K