2GGB
Novel bacterial methionine aminopeptidase inhibitors
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.722, 61.454, 53.308 |
| Unit cell angles | 90.00, 107.08, 90.00 |
Refinement procedure
| Resolution | 30.730 - 2.130 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gg0 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.360 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.730 | 2.250 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.021 | 0.082 |
| Number of reflections | 13433 | |
| <I/σ(I)> | 25.4 | 10.9 |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 2.7 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | 10 mg/ml protein, 25% PEG 8000, 100 mM TRIS-HCl, 1-5 mM inhibitor, batch, pH 7.0, temperature 298K |
