2GG6
CP4 EPSP synthase liganded with S3P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-09-16 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.151, 44.861, 77.177 |
| Unit cell angles | 90.00, 106.32, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.640 |
| R-factor | 0.158 |
| Rwork | 0.158 |
| R-free | 0.18900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gg4 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.630 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.700 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.064 | 0.380 |
| Number of reflections | 49732 | |
| <I/σ(I)> | 34.9 | 3.8 |
| Completeness [%] | 99.7 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 290 | (NH4)2SO4 / KCl / Tris-HCl /S3P, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
