2GG5
Novel bacterial methionine aminopeptidase inhibitors
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-01 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.776, 61.457, 53.902 |
| Unit cell angles | 90.00, 107.67, 90.00 |
Refinement procedure
| Resolution | 31.670 - 2.120 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gg2 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.387 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.670 | 2.250 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.042 | 0.294 |
| Number of reflections | 12996 | |
| <I/σ(I)> | 14.2 | 3.9 |
| Completeness [%] | 94.4 | 74.3 |
| Redundancy | 2.2 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | 10 mg/ml protein, 25% PEG 8000, 100 mM TRIS-HCl, 1-5 mM inhibitor, batch, pH 7.0, temperature 298K |
