2GFX

Structure of E. coli FabF(C163Q) in complex with Platensimycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	75.727, 75.727, 147.304
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	65.940 - 2.590
R-factor	0.1971
Rwork	0.194
R-free	0.25530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.297
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	BUSTER-TNT (1.1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.940	2.690
High resolution limit [Å]	2.590	2.590
Number of reflections	15670
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	19-24% PEG 8000, 0.1M Tris, 10mM BME, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K