2GFO

Structure of the Catalytic Domain of Human Ubiquitin Carboxyl-terminal Hydrolase 8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-09
Detector	SBC-3
Wavelength(s)	0.97896
Spacegroup name	P 61
Unit cell lengths	67.173, 67.173, 194.458
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.200 - 2.000
R-factor	0.17056
Rwork	0.168
R-free	0.21023
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.498
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.200	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.085
Number of reflections	33281
<I/σ(I)>	20.03
Completeness [%]	99.4	99.9
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	298	18% PEG3350; 0.1 M bis-Tris, pH 6.3, 0.2 M KSCN, 1 mM DTT., VAPOR DIFFUSION, HANGING DROP, temperature 298, temperature 298.0K