2GFN
Crystal structure of HTH-type transcriptional regulator pksA related protein from Rhodococcus sp. RHA1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-01-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795, 0.97934, 0.97948 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.475, 52.768, 59.818 |
| Unit cell angles | 90.00, 91.05, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.1752 |
| Rwork | 0.173 |
| R-free | 0.21482 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.402 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.097 | 0.560 |
| Number of reflections | 30702 | |
| <I/σ(I)> | 18.86 | 2.5 |
| Completeness [%] | 98.4 | 99.12 |
| Redundancy | 8.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 25% PEG2K MME, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
