2GFF

Crystal Structure of Yersinia pestis LsrG

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	70
Detector technology	CCD
Collection date	2005-07-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97910, 0.97951, 0.97444
Spacegroup name	P 41 21 2
Unit cell lengths	56.174, 56.174, 119.397
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.750
R-factor	0.20022
Rwork	0.197
R-free	0.25829
Structure solution method	MAD
RMSD bond length	0.021
RMSD bond angle	1.714
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.810
High resolution limit [Å]	1.750	1.750
Rmerge		0.680
Number of reflections	20024
<I/σ(I)>	11.4	1.9
Completeness [%]	99.3	98.9
Redundancy	8.5	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	295	20% PEG 6K, 100 mM MES, 0.17 M ammonium sulfate 100 microliter reservoir, 800 nanoliter drop,intelliplate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K