2GF7

Double tudor domain structure

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	CCD
Collection date	2004-08-13
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97930, 0.97990, 0.96000
Spacegroup name	H 3 2
Unit cell lengths	189.915, 189.915, 116.317
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.264
Rwork	0.225
R-free	0.26400
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.150	2.150
Rmerge	0.077	0.190
Number of reflections	43255	4029
<I/σ(I)>	13.4
Completeness [%]	99.2	92.9
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	290	1.4 M Ammonium Sulfate, 0.1 M Hepes (7.4), VAPOR DIFFUSION, HANGING DROP, temperature 290K
1	VAPOR DIFFUSION, HANGING DROP	7.4	290	1.4 M Ammonium Sulfate, 0.1 M Hepes (7.4), VAPOR DIFFUSION, HANGING DROP, temperature 290K
1	VAPOR DIFFUSION, HANGING DROP	7.4	290	1.4 M Ammonium Sulfate, 0.1 M Hepes (7.4), VAPOR DIFFUSION, HANGING DROP, temperature 290K