2GEZ
Crystal structure of potassium-independent plant asparaginase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.81 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.680, 60.200, 114.630 |
| Unit cell angles | 100.60, 92.90, 113.40 |
Refinement procedure
| Resolution | 25.000 - 2.600 |
| R-factor | 0.192 |
| Rwork | 0.189 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k2x |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.584 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.073 | 0.152 |
| Number of reflections | 34621 | |
| <I/σ(I)> | 14.3 | 6.1 |
| Completeness [%] | 99.0 | 96.9 |
| Redundancy | 2.8 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 20% PEG 4000, 100 mM HEPES, 200 mM MgCl2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292.0K |
