2GEY
Crystal Structure of AclR a putative hydroxylase from Streptomyces galilaeus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2005-06-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0202 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.908, 81.724, 136.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.980 - 1.800 |
| R-factor | 0.17594 |
| Rwork | 0.175 |
| R-free | 0.19712 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gex |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.096 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.200 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 79308 | |
| <I/σ(I)> | 17.3 | 3 |
| Completeness [%] | 98.2 | 88.2 |
| Redundancy | 7 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 18% PEG 400, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
