2GER
Crystal Structure and Oxidative Mechanism of Human Pyrroline-5-carboxylate Reductase
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-10 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 207.607, 123.805, 120.790 |
| Unit cell angles | 90.00, 121.76, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.100 |
| R-factor | 0.235 |
| Rwork | 0.233 |
| R-free | 0.26100 |
| Structure solution method | SAD |
| RMSD bond length | 1.466 |
| RMSD bond angle | 0.007 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.200 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 239396 | |
| Completeness [%] | 99.0 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.8-1M sodium acetate, 30-40mM imidazole, 50-60mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
