2GEQ
Crystal Structure of a p53 Core Dimer Bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.91756 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.755, 94.290, 119.822 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.150 - 2.300 |
| Rwork | 0.202 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tsr |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.350 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Number of reflections | 23284 | |
| <I/σ(I)> | 20.1 | 4.43 |
| Completeness [%] | 96.1 | 89.3 |
| Redundancy | 4.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 0.2 M NH4Cl, 0.01 M CaCl2, 0.05 M Tris-HCl, 28% Peg 4000 , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NH4Cl | ||
| 2 | 1 | 1 | CaCl2 | ||
| 3 | 1 | 1 | Tris-HCl | ||
| 4 | 1 | 1 | Peg 4000 | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | NH4Cl | ||
| 7 | 1 | 2 | CaCl2 | ||
| 8 | 1 | 2 | Peg 4000 | ||
| 9 | 1 | 2 | H2O |
