2GEM

2.1A crystal structure of Salmonella tyhpimurium YeaZ, a putative Gram-negative RPF, form-A

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-23
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.89843, 0.97925
Spacegroup name	P 32 2 1
Unit cell lengths	40.700, 40.700, 464.830
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.110 - 2.100
R-factor	0.22
Rwork	0.220
R-free	0.24800
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.036	0.327
Number of reflections	28024
<I/σ(I)>	28.3	2.3
Completeness [%]		44
Redundancy	3.3	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	22% PEG3350 + 0.2M Sodium Malonate, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 7.0
1	VAPOR DIFFUSION, SITTING DROP	7	293	22% PEG3350 + 0.2M Sodium Malonate, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 7.0