2GED
Signal Recognition Particle Receptor Beta-Subunit in nucleotide-free dimerized form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-16 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.00944 |
| Spacegroup name | P 61 |
| Unit cell lengths | 74.996, 74.996, 158.475 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.200 |
| R-factor | 0.18832 |
| Rwork | 0.183 |
| R-free | 0.23941 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1NRJ chain B |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.894 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BEAST |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 33.800 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 33373 |
| <I/σ(I)> | 24.2 |
| Completeness [%] | 98.6 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH UNDER PARAFFIN OIL | 5.5 | 277 | 1.3M ammonium sulfate, 50mM Bis/Tris/HCl, 0.5mM GDP, 5mM magnesium chloride, 5mM Hepes, 125mM sodium chloride, 2.5mM DTT, 0.25mM EDTA, pH 5.5, MICROBATCH UNDER PARAFFIN OIL, temperature 277K |
