2GDV
Sucrose phosphorylase from BIFIDOBACTERIUM ADOLESCENTIS reacted with sucrose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 2003-08-03 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.841 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.740, 103.070, 150.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.890 - 2.000 |
R-factor | 0.2 |
Rwork | 0.198 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1r7a |
RMSD bond length | 0.012 |
RMSD bond angle | 2.300 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.082 | 0.433 |
Number of reflections | 80388 | |
<I/σ(I)> | 21.5 | 2.6 |
Completeness [%] | 99.6 | 96.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG 4000, SODIUM ACETATE, TRIS, bicine, sucrose, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |