2GDR
Crystal structure of a bacterial glutathione transferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2005-04-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97974 |
| Spacegroup name | P 65 |
| Unit cell lengths | 103.440, 103.440, 262.280 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.1614 |
| Rwork | 0.161 |
| R-free | 0.20450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2GDH |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.061 | 0.384 |
| Number of reflections | 92159 | |
| Completeness [%] | 97.0 | 95.4 |
| Redundancy | 4.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 323 | 1M K/Na tartrate, 0.1 M MES, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 323K |
