2GDJ
Delta-62 RADA recombinase in complex with AMP-PNP and magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-09 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 |
| Unit cell lengths | 67.750, 67.750, 90.080 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| R-factor | 0.211 |
| Rwork | 0.210 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t4g |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.290 |
| Data reduction software | LSCALE |
| Data scaling software | LSCALE |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.620 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.052 | 0.309 |
| Number of reflections | 7410 | |
| <I/σ(I)> | 11.8 | 2.1 |
| Completeness [%] | 89.7 | 63.6 |
| Redundancy | 4.1 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 0.05 M MAGNESIUM CHLORIDE, 0.2 M NaCl, 0.1 M TRIS-HCL, 20% PEG 400, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K |
