2GDG
Crystal structure of covalently modified macrophage inhibitory factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 63 |
| Unit cell lengths | 94.705, 94.705, 87.674 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.450 |
| R-factor | 0.1485 |
| Rwork | 0.147 |
| R-free | 0.18304 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mff |
| RMSD bond length | 0.050 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 76879 | |
| Completeness [%] | 97.5 | 98.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 30% PEG 8000, 0.05M sodium phosphate buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
