2GD1

COENZYME-INDUCED CONFORMATIONAL CHANGES IN GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM BACILLUS STEAROTHERMOPHILLUS

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	83.800, 129.200, 83.100
Unit cell angles	90.00, 107.30, 90.00

Refinement procedure

Resolution	7.000 * - 2.500
R-factor	0.177
RMSD bond length	0.017
RMSD bond angle	0.041
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.098 *
Total number of observations	183964 *
Number of reflections	55743 *
Completeness [%]	95.4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	2	ammonium sulfate	2.33 (M)
2	1	2	Tris-HCl	10 (mM)
3	1	2	EDTA	2 (mM)
4	1	2	DTT
5	1	1	protein	5 (mg/ml)
6	1	1	ammonium sulfate	2.5 (M)