2GCS
Pre-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 181.199, 39.713, 71.038 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| Rwork | 0.221 |
| R-free | 0.24500 |
| Structure solution method | MIR |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.210 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 30830 | |
| Completeness [%] | 99.5 | 98.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 295 | 14% PEG 4000, 1M LiCl, 100mM MES-NaOH pH 5.6, 5mM MgCl2, 4mM GlcN6P, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 10 | 1 | 2 | GlcN6P | ||
| 2 | 1 | 1 | LiCl | ||
| 3 | 1 | 1 | MES-NaOH | ||
| 4 | 1 | 1 | MgCl2 | ||
| 5 | 1 | 1 | GlcN6P | ||
| 6 | 1 | 2 | PEG 4000 | ||
| 7 | 1 | 2 | LiCl | ||
| 8 | 1 | 2 | MES-NaOH | ||
| 9 | 1 | 2 | MgCl2 |
