2GCD

TAO2 kinase domain-staurosporine structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Wavelength(s)	1.5418
Spacegroup name	P 65 2 2
Unit cell lengths	186.019, 186.019, 94.641
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	160.130 - 2.550
R-factor	0.203
Rwork	0.199
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.033
RMSD bond angle	2.858
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	2.590
High resolution limit [Å]	2.550	6.880	2.550
Rmerge	0.075	0.028	0.580
Number of reflections	31295	1720	1354
<I/σ(I)>	11
Completeness [%]	97.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1