2GCA
apo form of L. casei FPGS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.070, 45.940, 85.380 |
| Unit cell angles | 90.00, 107.89, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| Rwork | 0.174 |
| R-free | 0.24840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | ROTAVATA |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.096 | 0.375 |
| Number of reflections | 13391 | |
| <I/σ(I)> | 7.3 | 2.1 |
| Completeness [%] | 84.2 | 88.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 277 | 40 mM acetate buffer, 4% PEG4000, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
