2GC5
G51S mutant of L. casei FPGS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.170, 45.830, 84.280 |
| Unit cell angles | 90.00, 107.66, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.850 |
| R-factor | 0.206 |
| Rwork | 0.204 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.284 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.073 | 0.390 |
| Number of reflections | 31920 | |
| <I/σ(I)> | 18.9 | 1.9 |
| Completeness [%] | 95.0 | 72.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 277 | 50 mM acetate and 16-24% PEG4000, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
