2GC0
The crystal structure of phosphoglucose isomerase from Pyrococcus furiosus in complex with 5-phospho-D-arabinonohydroxamate and zinc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-30 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.149, 73.112, 74.159 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.410 - 2.000 |
| R-factor | 0.169 |
| Rwork | 0.166 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x8e |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.216 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.159 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.116 | 0.420 |
| Total number of observations | 16233 | |
| Number of reflections | 25249 | 3597 |
| <I/σ(I)> | 5.6 | 1.8 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 4.6 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 0.1 M tri sodium citrate pH 6.5, 30% polyethylene glycol 4000 and 10% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
