2GBT
C6A/C111A CuZn Superoxide dismutase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.092 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 156.618, 35.257, 114.971 |
| Unit cell angles | 90.00, 112.17, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.204 |
| Rwork | 0.203 |
| R-free | 0.23000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1hl4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.031 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.059 | 0.378 |
| Number of reflections | 64389 | |
| <I/σ(I)> | 1.8 | |
| Completeness [%] | 99.4 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 18% PEG8000, 50mM TRIS-HCL, 50mM NaAc, 1mM EDTA, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
