2GBO
Protein of Unknown Function EF2458 from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97933 |
| Spacegroup name | P 62 |
| Unit cell lengths | 54.614, 54.614, 142.121 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.500 - 2.200 |
| R-factor | 0.1989 |
| Rwork | 0.199 |
| R-free | 0.24360 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.535 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.400 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.104 | 0.669 |
| Number of reflections | 12040 | |
| <I/σ(I)> | 34.5 | 1.83 |
| Completeness [%] | 98.7 | 91.2 |
| Redundancy | 15.9 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 35% (v/v) 2-methyl-2,4-pentanediol, 0.1 M HEPES, 0.2 M NaCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
