2GBN
Crystal Structure of the 35-36 8 Glycine Insertion Mutant of Ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00808 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 45.773, 45.773, 111.549 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.350 - 1.600 |
| R-factor | 0.20591 |
| Rwork | 0.203 |
| R-free | 0.26491 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gbm |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.947 |
| Data scaling software | d*TREK (9.1L) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.350 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.094 | 0.654 |
| Total number of observations | 13241 | |
| Number of reflections | 9821 | 942 |
| <I/σ(I)> | 16.5 | 3.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.17 | 14.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 279.15 | 25-30% PEG 4000, 50 mM Sodium Cacodylate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 279.15K |
