2GBA
Reduced Cu(I) form at pH 4 of P52G mutant of amicyanin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.450, 56.132, 27.003 |
| Unit cell angles | 90.00, 96.98, 90.00 |
Refinement procedure
| Resolution | 20.000 - 0.920 |
| R-factor | 0.1076 |
| R-free | 0.14750 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.032 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 0.950 |
| High resolution limit [Å] | 0.920 | 0.920 |
| Rmerge | 0.037 | 0.214 |
| Number of reflections | 40193 | |
| <I/σ(I)> | 32.6 | |
| Completeness [%] | 68.5 | 50.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.3 | 293 | 3.0 M sodium phosphate 90:10 monobasic:dibasic covered drop in mineral oil, pH 4.3, EVAPORATION, temperature 293K |
