2G9W

Crystal Structure of Rv1846c, a Putative Transcriptional Regulatory Protein of Mycobacterium Tuberculosis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	110
Detector technology	CCD
Collection date	2005-03-11
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97919, 0.93929, 0.97935
Spacegroup name	P 21 21 21
Unit cell lengths	60.580, 64.330, 75.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.800
R-factor	0.20444
Rwork	0.202
R-free	0.25201
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.415
Data reduction software	XDS
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.050	0.493
Number of reflections	27947
<I/σ(I)>	11.7	2.3
Completeness [%]	99.5	99.4
Redundancy	3.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	291	1.6M sodium chloride, 50mM magnesium chloride, 100mM sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K