2G9Q
The crystal structure of the glycogen phosphorylase b- 1AB complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-02-11 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.712, 128.712, 117.442 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.690 - 2.500 |
| R-factor | 0.19242 |
| Rwork | 0.190 |
| R-free | 0.24276 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1hlf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.199 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.690 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 31719 | |
| <I/σ(I)> | 10 | 3.3 |
| Completeness [%] | 91.3 | 93.7 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 287 | BES, EDTA, pH 6.7, SMALL TUBES, temperature 287K |
