2G9F
Crystal structure of intein-tagged mouse PNGase C-terminal domain
Experimental procedure
| Experimental method | SIRAS |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0055 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 40.760, 40.760, 193.663 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.16895 |
| Rwork | 0.167 |
| R-free | 0.21265 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.413 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.091 | 0.622 |
| Number of reflections | 15560 | |
| <I/σ(I)> | 36.4 | 3.5 |
| Completeness [%] | 99.9 | 98.6 |
| Redundancy | 9.4 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 14-16% PEG 8000, 100 mM Tris-HCl, pH 8.5, 120 mM MgCl2 and 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
