2G9D
Crystal Structure of Succinylglutamate desuccinylase from Vibrio cholerae, Northeast Structural Genomics Target VcR20
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-01-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97929, 0.97944, 0.96794 |
| Spacegroup name | P 65 |
| Unit cell lengths | 82.934, 82.934, 116.665 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.940 - 3.000 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.27200 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.940 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.069 | 0.332 |
| Number of reflections | 17624 | |
| <I/σ(I)> | 24.49 | 6.1 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 6.2 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 291 | 100 mM MOPS, 2880 mM Potassium Acetate, 5 mM DDT, pH 7.0, Microbatch, temperature 291K |
