2G98
human gamma-D-crystallin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Collection date | 1999-06-16 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.375, 81.778, 106.279 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.900 - 2.200 |
| R-factor | 0.20034 |
| Rwork | 0.197 |
| R-free | 0.25735 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1elp |
| RMSD bond length | 0.037 |
| RMSD bond angle | 2.687 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.620 | |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.062 | 0.230 |
| Number of reflections | 21417 | |
| Completeness [%] | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Several crystals, ~0.15 x 0.15 x 0.5 mm from the lens tissue extract were transferred to PBS buffer with 25% (v/v) glycerol and flash cooled in 100 K nitrogen stream for measurements. |
