2G8Y
The structure of a putative malate/lactate dehydrogenase from E. coli.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-01 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97923, 0.97937, 1.00314 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 139.095, 139.095, 151.654 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.500 - 2.150 |
| R-factor | 0.15902 |
| Rwork | 0.158 |
| R-free | 0.18622 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.476 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.500 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 88697 | |
| Completeness [%] | 96.1 | 72.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 302 | 1.8M ammonium sulfate, 0.1M sodium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 302K |
