2G8U

B. halodurans RNase H catalytic domain D132N mutant in complex with Mg2+ and RNA/DNA hybrid (non-P nick at the active site)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2005-06-06
Detector	RIGAKU RAXIS IV
Spacegroup name	C 1 2 1
Unit cell lengths	83.878, 35.412, 63.839
Unit cell angles	90.00, 98.66, 90.00

Refinement procedure

Resolution	30.000 - 2.700
Rwork	0.223
R-free	0.28800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zbi
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.820
High resolution limit [Å]	2.700	2.700
Number of reflections	4718
Completeness [%]	89.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	278	25% MPD, 0.2M NaCl, 0.1M HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 278K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	MPD
2	1	1	NaCl
3	1	1	HEPES
4	1	1	H2O
5	1	2	MPD
6	1	2	NaCl
7	1	2	HEPES