2G8L

Crystal structure of a duf89 family protein (ph1575) from pyrococcus horikoshii at 2.04 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-15
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000, 0.9796, 0.9797
Spacegroup name	P 31
Unit cell lengths	88.859, 88.859, 81.499
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.010 - 2.040
R-factor	0.146
Rwork	0.144
R-free	0.18200
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.345
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.760		2.110
High resolution limit [Å]	2.040	3.480	2.040
Rmerge	0.067	0.048	0.437
Number of reflections	45685	4589	3925
<I/σ(I)>	12.28	21.9	2.93
Completeness [%]	99.7	99.8	96.9
Redundancy	3.78		3.39

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	4	277	10.0% MPD, 0.1M Citrate, pH 4.0, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K