2G8K

B. halodurans RNase H catalytic domain D192N mutant in complex with Ca2+ and RNA/DNA hybrid (non-P nick at the active site)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2005-09-14
Detector	RIGAKU RAXIS IV
Spacegroup name	C 1 2 1
Unit cell lengths	82.052, 36.925, 61.860
Unit cell angles	90.00, 96.30, 90.00

Refinement procedure

Resolution	30.000 - 1.650
Rwork	0.182
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zbi
RMSD bond length	0.004
RMSD bond angle	1.150
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.710
High resolution limit [Å]	1.650	1.650
Number of reflections	20635
Completeness [%]	91.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	278	27% MPD, 0.25M ammonium acetate, Na Citrate pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 278K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	MPD
2	1	1	ammonium acetate
3	1	1	sodium Citrate
4	1	1	H2O
5	1	2	MPD
6	1	2	ammonium acetate
7	1	2	sodium Citrate