2G8D
Lactobacillus casei thymidylate synthase Y261W-dUMP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 1996-01-01 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 78.720, 78.720, 242.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.470 - 2.400 |
R-factor | 0.186 |
Rwork | 0.186 |
R-free | 0.25100 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.026 |
RMSD bond angle | 2.400 |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.500 | 2.550 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.088 | |
Number of reflections | 16205 | |
<I/σ(I)> | 11 | 2.3 |
Completeness [%] | 88.7 | 76.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | Vapor diffusion against 20mM KPO4 buffer from protein solutions in the same buffer, containing 15-20mM ammonium sulfate, DTT and 38mM dUMP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.K |