2G85
Crystal structure of chorismate synthase from Mycobacterium tuberculosis at 2.22 angstrons of resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-05 |
| Detector | RIGAKU |
| Wavelength(s) | 1.427 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 130.288, 130.288, 157.950 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.730 - 2.220 |
| R-factor | 0.1732 |
| Rwork | 0.173 |
| R-free | 0.21441 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ztb |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.286 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.250 | 2.340 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Number of reflections | 37564 | |
| Completeness [%] | 99.4 | 22 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 0.1M Hepes-Na pH 7.8 buffer, 4M sodim chlorate , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
