2G7U
2.3 A structure of putative catechol degradative operon regulator from Rhodococcus sp. RHA1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-11 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97918, 0.97934 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 96.407, 186.520, 126.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 93.250 - 2.300 |
| R-factor | 0.18261 |
| Rwork | 0.180 |
| R-free | 0.23123 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.347 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.250 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.084 | 0.476 |
| Number of reflections | 50831 | |
| <I/σ(I)> | 33.4 | 3.88 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.1 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 25% PEG3350, 0.2M Li Sulphate, 0.1M Bis-Tris, cryo condition is 8/8/8% (Sucr,Glycer,550MME)in mag. sol., pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
