2G7S
The crystal structure of transcriptional regulator, TetR family, from Agrobacterium tumefaciens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-03 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9793164 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 47.736, 55.029, 73.979 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.400 |
| R-factor | 0.13896 |
| Rwork | 0.136 |
| R-free | 0.18605 |
| Structure solution method | SAD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.068 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 38903 | |
| <I/σ(I)> | 35 | 2.8 |
| Completeness [%] | 99.7 | 97.5 |
| Redundancy | 6.9 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.5M K3PO4,0.5M Na3PO4 , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
