2G7O

Protonation-mediated structural flexibility in the F conjugation regulatory protein, TraM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	105
Detector technology	CCD
Collection date	2005-06-14
Detector	ADSC QUANTUM 210
Wavelength(s)	1.11697
Spacegroup name	I 4
Unit cell lengths	51.733, 51.733, 49.585
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.580 - 1.400
R-factor	0.195
Rwork	0.193
R-free	0.22400
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.233
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.08)
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.580	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.039	0.371
Number of reflections	12828	548
<I/σ(I)>	27.6	3.12
Completeness [%]	99.1	86.6
Redundancy	3.6	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	297	30% PEG 400, 0.1M MES pH 6.5, 2mM DTT, 0.5M ammonium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 297K
1	VAPOR DIFFUSION, HANGING DROP	6.5	297	30% PEG 400, 0.1M MES pH 6.5, 2mM DTT, 0.5M ammonium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 297K
1	VAPOR DIFFUSION, HANGING DROP	6.5	297	30% PEG 400, 0.1M MES pH 6.5, 2mM DTT, 0.5M ammonium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 297K