2G7L
Crystal structure of putative transcription regulator SCO7704 from Streptomyces coelicor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-26 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.969 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 96.099, 96.099, 59.096 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.22815 |
| Rwork | 0.225 |
| R-free | 0.28587 |
| Structure solution method | SAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.924 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 16602 | |
| <I/σ(I)> | 32 | 4 |
| Completeness [%] | 99.0 | 93 |
| Redundancy | 13.5 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 4M NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
