2G7F
The 1.95 A crystal structure of Vibrio cholerae extracellular endonuclease I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-17 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.400, 64.750, 75.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.650 - 1.950 |
| R-factor | 0.179 |
| Rwork | 0.177 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ouo |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.446 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.646 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.075 | 0.378 |
| Total number of observations | 2045 | |
| Number of reflections | 13324 | 1019 |
| <I/σ(I)> | 9.2 | 2 |
| Completeness [%] | 88.8 | 49 |
| Redundancy | 3.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 293 | 30% PEG 8000 w/v, 0.2 M Na acetate, 0.1 M cacodylate buffer, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
