2G74
Y104F mutant of type 1 isopentenylpyrophosphate-dimethylallylpyrophosphate isomerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-06-30 |
Detector | MARRESEARCH |
Wavelength(s) | 1.54179 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.935, 71.477, 91.955 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.960 |
R-factor | 0.201 |
Rwork | 0.201 |
R-free | 0.26950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hx3 |
RMSD bond length | 0.021 |
RMSD bond angle | 0.022 |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.030 |
High resolution limit [Å] | 1.960 | 1.960 |
Rmerge | 0.054 | 0.226 |
Number of reflections | 30195 | |
<I/σ(I)> | 5.1 | |
Completeness [%] | 99.3 | 93.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | PEG 2000MME, MANGANESE AND MAGNESIUM CHLORIDE, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |