2G67
E. Coli Pyruvate Dehydrogenase E1 Component (Apoenzyme)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 93 |
| Detector technology | AREA DETECTOR |
| Collection date | 2001-02-23 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.610, 140.630, 81.910 |
| Unit cell angles | 90.00, 102.87, 90.00 |
Refinement procedure
| Resolution | 34.620 - 2.320 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.28100 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 1l8a |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.546 |
| Data reduction software | FBSCALE |
| Data scaling software | X-GEN |
| Phasing software | PHASES |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.620 | 2.400 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.060 | |
| Number of reflections | 71362 | |
| <I/σ(I)> | 24.01 | 6.6 |
| Completeness [%] | 90.5 | 44.1 |
| Redundancy | 2.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | PEG2000 MONOMETHYL ETHER, 2-PROPANOL, SODIUM AZIDE, HEPES BUFFER, PH 7.00, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 291K |
