2G67

E. Coli Pyruvate Dehydrogenase E1 Component (Apoenzyme)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	93
Detector technology	AREA DETECTOR
Collection date	2001-02-23
Detector	SIEMENS HI-STAR
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	81.610, 140.630, 81.910
Unit cell angles	90.00, 102.87, 90.00

Refinement procedure

Resolution	34.620 - 2.320
R-factor	0.212
Rwork	0.212
R-free	0.28100
Structure solution method	DIFFERENCE FOURIER
Starting model (for MR)	1l8a
RMSD bond length	0.010
RMSD bond angle	1.546
Data reduction software	FBSCALE
Data scaling software	X-GEN
Phasing software	PHASES
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.620	2.400
High resolution limit [Å]	2.310	2.310
Rmerge	0.060
Number of reflections	71362
<I/σ(I)>	24.01	6.6
Completeness [%]	90.5	44.1
Redundancy	2.85

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	PEG2000 MONOMETHYL ETHER, 2-PROPANOL, SODIUM AZIDE, HEPES BUFFER, PH 7.00, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 291K