2G66
Crystal structure of a collagen-like peptide with 3(S)Hyp in the Xaa position
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.989 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 18.120, 19.450, 81.500 |
| Unit cell angles | 90.00, 91.26, 90.00 |
Refinement procedure
| Resolution | 18.920 - 1.800 |
| R-factor | 0.275 |
| Rwork | 0.272 |
| R-free | 0.29200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k6f |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.900 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.500 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.104 | |
| Number of reflections | 5300 | |
| Completeness [%] | 97.1 | 94.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 25% PEG 4000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
