2G5X

Crystal structure of lychnin a type 1 Ribosome Inactivating Protein (RIP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	CCD
Collection date	2002-12-17
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	35.247, 57.333, 53.230
Unit cell angles	90.00, 106.25, 90.00

Refinement procedure

Resolution	28.440 - 1.700
R-factor	0.162
Rwork	0.162
R-free	0.19600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1WUC
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.100	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	21895
<I/σ(I)>	39.7	14.2
Completeness [%]	51.0	50.2
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	30% PEG 8000, 0.1M sodium phosphate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K