2G5K

Crystal Structure of the Homo sapiens Cytoplasmic Ribosomal Decoding Site complexed with Apramycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2003-05-18
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9793
Spacegroup name	P 31
Unit cell lengths	33.123, 33.123, 116.421
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.800
Rwork	0.258
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.900
Data reduction software	CrystalClear ((MSC/RIGAKU))
Data scaling software	CrystalClear ((MSC/RIGAKU))
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.850	27.850	2.900
High resolution limit [Å]	2.800	6.010	2.800
Rmerge	0.056	0.038	0.397
Total number of observations		1886	1993
Number of reflections	3533	364	346
<I/σ(I)>	16.8	45.5	2.1
Completeness [%]	100.0
Redundancy	5.59	5.01	5.76

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	300	50mM sodium cacodylate, 10mM hexammine cobalt chloride, 150mM potassium chloride, 1%(v/v) 2-methyl-2,4-pentanediol, 5%(v/v) glycerol, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 300K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	sodium cacodylate
10	1	2	glycerol
11	1	2	H2O
2	1	1	hexammine cobalt chloride
3	1	1	potassium chloride
4	1	1	2-methyl-2,4-pentanediol
5	1	1	glycerol
6	1	1	H2O
7	1	2	sodium cacodylate
8	1	2	hexammine cobalt chloride
9	1	2	potassium chloride